DNA quantitation

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Subject Bio Chem

Spectrometry, micropipette, dilutions, quantitation, quality checks, controls.

Background         
DNA is easily quantitated and quality checked for co-purified protein using UV spectrometry. Using free-choice dilution options, this instrument allows students to QC and estimate DNA concentration of 5 genomic DNAs.

The guide below outlines the three activities available which take you through the stages involved in checking the purity of a genomic DNA sample and estimating its concentration.

Duration
Full creation of suitable dilution series and data collection from 5 DNA samples will take up to 2 hours.

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